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Just a note

In this example the data have been put into groups of 5. Note the median trace should not be viewed as an alternative to model fitting (see below), but as a valuable indicator of which is the most appropriate model (or family of models) to investigate. This is a U-shaped relationship, so you have the option of fitting either:
  • two separate linear or curvilinear relationships to model each 'arm' of the U or
  • a polynomial function. These can be used to model almost any shape of relationship, but have the disadvantage that it is difficult to attach any biological meaning to the various parameters.