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Just a note
Plotting the cumulative number of species observed against time (or area sampled) produces a curve. As sampling proceeds the number of new species found flattens off  or reaches an 'asymptote'. Hence a simple way of estimating the total number of species in a community is to decide at what point the line would level off completely.
One obvious difficulty with this is the number of species you observe is partly a matter of chance and season  which introduces a series of arbitrary steps into your graph. The effect of this can be reduced by randomly reordering the sequence in which species were identified  then reestimating the asymptote. By repeating this process a number of times a mean asymptote can be estimated, and its standard deviation calculated. Needless to say, estimating the total number of species in a community from an asymptote conceals some rather crucial assumptions...
